In the hucgfp zebrafish, there were no significant differences in the length of the axon extending from the trunk between control, negative control, and 0. Serotonin promotes development and regeneration of spinal motor neurons in zebrafish. Hucgfp and olig2 dsred zebrafish models which have fluorescenceexpressing cns were used to analyze the effect of tcs on neural development. Adult zebrafish, in contrast to mammals, regenerate neurons in their brain, but the extent and variability of this capacity is unclear. A caged ret kinase inhibitor and its effect on motoneuron. B activation in the zebrafish digestive tract michelle kanther, 1, 2 xiaolun sun, 3 marcus muhlbauer, 3 lantz c. The zebrafish embryo is especially valuable for cell biological studies because of its. The zebrafish danio rerio is a freshwater fish belonging to the minnow family cyprinidae of the order cypriniformes. Threecolor imaging using fluorescent proteins in living zebrafish. Flynn, iii, 1 michel bagnat, 4 christian jobin, 3, 5, 6 and john f. Microbial colonization induces dynamic temporal and. A a confocal image representing the 4 dpf double transgenic zebrafish embryo. Triclosan affects axon formation in the neural development.
Trimethyltin chloride c3h9clsn, tmt is a toxic organotin byproduct occurring in the worldwide production of plastic in industry and agriculture. It has been widely used as a pesticide, preservative, coating, and light and heat stabilizer for making polyvinyl chloride pvc. Analyses of double transgenic zebrafish embryos tggata1. Zebrafish are omnivorous, feeding on small crustacea, insect larvae, and algae. The zebrafish information network zfin is the database of genetic and genomic data for the zebrafish danio rerio as a model organism. Using zebrafish to model erythroid lineage toxicity and regeneration. Reporters using cathepsin k promoter as ctskdsred 69. Several pathological models have been established in adult zebrafish such as bone injury models. Larvae largely hunt prey paramecia visually and darkness impairs this. Please use this form to recommend updates to the information in zfin. Tmt affected the fluorescent cns structure by decreasing pattern of hucgfp and olig2 dsred transgenic zebrafish. A guide for the laboratory use of zebrafish danio brachydanio rerio.
Product description type unit quantity academic price book. Fluorescent imaging demonstrates that gfp and dsred are highly stable. A guide for the laboratory use of zebrafish danio rerio, eugene, university of. Tmt affected the fluorescent cns structure by decreasing pattern of hucgfp and olig2dsred transgenic zebrafish. Zebrafish danio rerio as a model organism intechopen. In addition, it significantly modulated the expression patterns of sonic hedgehog a shha, neurogenin1 ngn1, embryonic lethal abnormal vision like protein 3 elavl3, and glial fibrillary acidic protein gfap. Fish, affected genomic regions, phenotype, gene expression. Here we ask whether the loss of various dopaminergic neuron populations is sufficient to trigger their functional regeneration. Culturing zebrafish larvae in tnbs from 3 days postfertilization dpf through 8 dpf has been shown to elicit a broad but disparate range of intestinal phenotypes.
Microbial colonization induces dynamic temporal and spatial patterns of nf. Zebrafish appear to habituate to their surroundings, including vibrations such as water pumps, but react strongly to sudden noise and vibration, which should be avoided. Danio rerio zebrafish is an elective model organism for the study of vertebrate. Regeneration of dopaminergic neurons in adult zebrafish. In zebrafish, intersectional control of transgene expression has been achieved by. In addition, the zebrafish is increasingly used as a genetic model organism for. Tnbs, a haptenizing agent, is thought to render colonic proteins immunogenic to the host organism and has been a standard tool for induction of intestinal inflammation in mice. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext.
We used regulatory regions of the zebrafish lysozyme c lysc gene to. The zebrafish in biomedical research 1st edition elsevier. Purchase the zebrafish in biomedical research 1st edition. However, relatively few studies have estimated the effects of tmt toxicity on neurodevelopment. Among the model organisms, the zebrafish danio rerio is one of the best leading. The zebrafish lysozyme c promoter drives myeloidspecific. Brainwide cellular resolution imaging of cre transgenic zebrafish. Pdf serotonin promotes development and regeneration of. We appreciate as much detail as possible and references as appropriate.
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